Elucidation of amidating reaction mechanism by frog amidating enzyme, peptidylglycine alpha-hydroxylating monooxygenase, expressed in insect cell culture.

A frog 'peptidylglycine alpha-amidating monooxygenase (PAM, EC 1.14.17.3)' was expressed in cultured insect cells by using the baculovirus expression vector system. The enzyme, recovered in the culture medium, was purified to homogeneity. Its apparent molecular mass (43 kd), estimated by b...

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Main Authors: Suzuki, K, Shimoi, H, Iwasaki, Y, Kawahara, T, Matsuura, Y, Nishikawa, Y
Formato: Artigo
Idioma:Inglês
Publicado: 1990
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC552208/
https://ncbi.nlm.nih.gov/pubmed/2265607
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