Elucidation of amidating reaction mechanism by frog amidating enzyme, peptidylglycine alpha-hydroxylating monooxygenase, expressed in insect cell culture.

A frog 'peptidylglycine alpha-amidating monooxygenase (PAM, EC 1.14.17.3)' was expressed in cultured insect cells by using the baculovirus expression vector system. The enzyme, recovered in the culture medium, was purified to homogeneity. Its apparent molecular mass (43 kd), estimated by both SDS-PA...

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Bibliografische gegevens

Gepubliceerd in:	EMBO J
Hoofdauteurs:	Suzuki, K, Shimoi, H, Iwasaki, Y, Kawahara, T, Matsuura, Y, Nishikawa, Y
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Nature Publishing Group 1990
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552208/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2265607/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07874.x
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