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Mapping the active site of ribonuclease P RNA using a substrate containing a photoaffinity agent.

Ribonuclease P RNA is the catalytic moiety of the ribonucleoprotein enzyme that removes precursor sequences from 5'-ends of pre-tRNAs. A photoaffinity cross-linking agent was coupled to the substrate phosphate on which RNase P acts and used to map nucleotides in the vicinity of the catalytic si...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Burgin, A B, Pace, N R
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552184/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1701142/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07633.x
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