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Mapping the active site of ribonuclease P RNA using a substrate containing a photoaffinity agent.
Ribonuclease P RNA is the catalytic moiety of the ribonucleoprotein enzyme that removes precursor sequences from 5'-ends of pre-tRNAs. A photoaffinity cross-linking agent was coupled to the substrate phosphate on which RNase P acts and used to map nucleotides in the vicinity of the catalytic si...
Gardado en:
| Publicado en: | EMBO J |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Nature Publishing Group
1990
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552184/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1701142/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07633.x |
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