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Mapping the active site of ribonuclease P RNA using a substrate containing a photoaffinity agent.

Ribonuclease P RNA is the catalytic moiety of the ribonucleoprotein enzyme that removes precursor sequences from 5'-ends of pre-tRNAs. A photoaffinity cross-linking agent was coupled to the substrate phosphate on which RNase P acts and used to map nucleotides in the vicinity of the catalytic si...

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Autors principals: Burgin, A B, Pace, N R
Format: Artigo
Idioma:Inglês
Publicat: 1990
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC552184/
https://ncbi.nlm.nih.gov/pubmed/1701142
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