Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.
The EcoRI restriction endonuclease cleaves DNA molecules at the sequence GAATTC. We devised a genetic screen to isolate EcoRI mutants with altered or broadened substrate specificity. In vitro, the purified mutant enzymes cleave both the wild-type substrate and sites which differ from this by one nuc...
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| Published in: | EMBO J |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Nature Publishing Group
1990
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552075/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2209548/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07538.x |
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