One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs

The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited animals showed mosaicism, with editing occurring only in a portion of cells. Here we show that single gene or multiple genes can be completely knocked out in mouse and monkey embryos by zygotic injection of C...

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Detalhes bibliográficos
Publicado no:Cell Res
Main Authors: Zuo, Erwei, Cai, Yi-Jun, Li, Kui, Wei, Yu, Wang, Bang-An, Sun, Yidi, Liu, Zhen, Liu, Jiwei, Hu, Xinde, Wei, Wei, Huo, Xiaona, Shi, Linyu, Tang, Cheng, Liang, Dan, Wang, Yan, Nie, Yan-Hong, Zhang, Chen-Chen, Yao, Xuan, Wang, Xing, Zhou, Changyang, Ying, Wenqin, Wang, Qifang, Chen, Ren-Chao, Shen, Qi, Xu, Guo-Liang, Li, Jinsong, Sun, Qiang, Xiong, Zhi-Qi, Yang, Hui
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5518993/
https://ncbi.nlm.nih.gov/pubmed/28585534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/cr.2017.81
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