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3D single-molecule tracking enables direct hybridization kinetics measurement in solution

Single-molecule measurements of DNA hybridization kinetics are mostly performed on a surface or inside a trap. Here we demonstrate a time-resolved, 3D single-molecule tracking (3D-SMT) method that allows us to follow a freely diffusing ssDNA molecule in solution for hundreds of milliseconds or even...

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Bibliografiske detaljer
Udgivet i:Nanoscale
Main Authors: Liu, Cong, Obliosca, Judy M., Liu, Yen-Liang, Chen, Yu-An, Jiang, Ning, Yeh, Hsin-Chih
Format: Artigo
Sprog:Inglês
Udgivet: 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5515391/
https://ncbi.nlm.nih.gov/pubmed/28422238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7nr01369h
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