Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS

Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach that combines segmental isotope labeling of RNA with photo-crosslinking and tandem mass spectrometry to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. Th...

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Publié dans:Nat Methods
Auteurs principaux: Dorn, G., Leitner, A., Boudet, J., Campagne, S., von Schroetter, C., Moursy, A., Aebersold, R., Allain, F. H.-T.
Format: Artigo
Langue:Inglês
Publié: 2017
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5505470/
https://ncbi.nlm.nih.gov/pubmed/28346450
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4235
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