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Structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and MS/MS
Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach that combines segmental isotope labeling of RNA with photo-crosslinking and tandem mass spectrometry to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. Th...
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| Publicado no: | Nat Methods |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5505470/ https://ncbi.nlm.nih.gov/pubmed/28346450 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4235 |
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