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CRISPR/Cas9 system-mediated impairment of synaptobrevin/VAMP function in postmitotic hippocampal neurons

BACKGROUND: The use of the CRISPR/Cas9 system is becoming widespread, however current studies have predominantly focused on dividing cells. It is currently unknown if CRISPR/Cas9 can be used in a postmitotic setting to examine non-cell autonomous/presynaptic phenotypes in the resulting genetically h...

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Bibliografische gegevens
Gepubliceerd in:J Neurosci Methods
Hoofdauteurs: Horvath, Patricia M., Kavalali, Ege T., Monteggia, Lisa M.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2016
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5505177/
https://ncbi.nlm.nih.gov/pubmed/28025172
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2016.12.015
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