Base-Pair Resolution Genome-Wide Mapping Of Active RNA polymerases using Precision Nuclear Run-On (PRO-seq)

We provide a protocol for precision nuclear run-on sequencing (PRO-seq) and its variant, PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription start sites (TSSs) (PRO-cap) genome-wide at high resolution. The density of RNA polymerases at a particular genomic locus dire...

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Publicado en:Nat Protoc
Autores principales: Mahat, Dig Bijay, Kwak, Hojoong, Booth, Gregory T., Jonkers, Iris H., Danko, Charles G., Patel, Ravi K., Waters, Colin T., Munson, Katie, Core, Leighton J., Lis, John T.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2016
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5502525/
https://ncbi.nlm.nih.gov/pubmed/27442863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.086
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