DNA isolation protocol effects on nuclear DNA analysis by microarrays, droplet digital PCR, and whole genome sequencing, and on mitochondrial DNA copy number estimation

Potential bias introduced during DNA isolation is inadequately explored, although it could have significant impact on downstream analysis. To investigate this in human brain, we isolated DNA from cerebellum and frontal cortex using spin columns under different conditions, and salting-out. We first a...

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Dades bibliogràfiques
Publicat a:PLoS One
Autors principals: Nacheva, Elizabeth, Mokretar, Katya, Soenmez, Aynur, Pittman, Alan M., Grace, Colin, Valli, Roberto, Ejaz, Ayesha, Vattathil, Selina, Maserati, Emanuela, Houlden, Henry, Taanman, Jan-Willem, Schapira, Anthony H., Proukakis, Christos
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2017
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5500342/
https://ncbi.nlm.nih.gov/pubmed/28683077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0180467
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