DNA isolation protocol effects on nuclear DNA analysis by microarrays, droplet digital PCR, and whole genome sequencing, and on mitochondrial DNA copy number estimation

Potential bias introduced during DNA isolation is inadequately explored, although it could have significant impact on downstream analysis. To investigate this in human brain, we isolated DNA from cerebellum and frontal cortex using spin columns under different conditions, and salting-out. We first a...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Nacheva, Elizabeth, Mokretar, Katya, Soenmez, Aynur, Pittman, Alan M., Grace, Colin, Valli, Roberto, Ejaz, Ayesha, Vattathil, Selina, Maserati, Emanuela, Houlden, Henry, Taanman, Jan-Willem, Schapira, Anthony H., Proukakis, Christos
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5500342/
https://ncbi.nlm.nih.gov/pubmed/28683077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0180467
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