Initiation of rolling-circle replication in pT181 plasmid: initiator protein enhances cruciform extrusion at the origin.
Plasmid pT181 DNA secondary structures have been analyzed in vitro by nuclease S1 digestion and in vivo by bromoacetaldehyde treatment. A cruciform structure occurring at the pT181 replication origin in vitro is greatly enhanced by the binding of the plasmid-encoded initiator protein RepC. In vivo a...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54996/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2236066/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.21.8560 |
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