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Functional characterization and architecture of recombinant yeast SWR1 histone exchange complex
We have prepared recombinant fourteen subunit yeast SWR1 complex from insect cells using a modified MultiBac system. The 1.07 MDa recombinant protein complex has histone-exchange activity. Full exchange activity is realized with a single SWR1 complex bound to a nucleosome. We also prepared mutant co...
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Publicado no: | Nucleic Acids Res |
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Main Authors: | , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5499540/ https://ncbi.nlm.nih.gov/pubmed/28499038 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx414 |
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