Functional characterization and architecture of recombinant yeast SWR1 histone exchange complex

We have prepared recombinant fourteen subunit yeast SWR1 complex from insect cells using a modified MultiBac system. The 1.07 MDa recombinant protein complex has histone-exchange activity. Full exchange activity is realized with a single SWR1 complex bound to a nucleosome. We also prepared mutant co...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Lin, Chia-Liang, Chaban, Yuriy, Rees, David M., McCormack, Elizabeth A., Ocloo, Lorraine, Wigley, Dale B.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2017
Subjects:
Genome Integrity, Repair and Replication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5499540/
https://ncbi.nlm.nih.gov/pubmed/28499038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx414
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