Polyproline and the “spectroscopic ruler” revisited with single-molecule fluorescence

To determine whether Förster resonance energy transfer (FRET) measurements can provide quantitative distance information in single-molecule fluorescence experiments on polypeptides, we measured FRET efficiency distributions for donor and acceptor dyes attached to the ends of freely diffusing polypro...

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Main Authors: Schuler, Benjamin, Lipman, Everett A., Steinbach, Peter J., Kumke, Michael, Eaton, William A.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2005
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC549440/
https://ncbi.nlm.nih.gov/pubmed/15699337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0408164102
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