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Effect of flexibility and cis residues in single-molecule FRET studies of polyproline

Polyproline has recently been used as a spacer between donor and acceptor chromophores to help establish the accuracy of distances determined from single-molecule Förster resonance energy transfer (FRET) measurements. This work showed that the FRET efficiency in water is higher than expected for a r...

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Bibliographic Details
Main Authors: Best, Robert B., Merchant, Kusai A., Gopich, Irina V., Schuler, Benjamin, Bax, Ad, Eaton, William A.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2007
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2141891/
https://ncbi.nlm.nih.gov/pubmed/18029448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0709567104
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