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Fluorescence Quenching by TEMPO: A Sub-30 Å Single-Molecule Ruler

A series of DNA molecules labeled with 5-carboxytetramethylrhodamine (5-TAMRA) and the small nitroxide radical TEMPO were synthesized and tested to investigate whether the intramolecular quenching efficiency can be used to measure short intramolecular distances in small ensemble and single-molecule...

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Sonraí Bibleagrafaíochta
Main Authors: Zhu, Peizhi, Clamme, Jean-Pierre, Deniz, Ashok A.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Biophysical Society 2005
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366861/
https://ncbi.nlm.nih.gov/pubmed/16199509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.071027
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