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Fluorescence quenching: A tool for single-molecule protein-folding study

By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye molecules attached are able to fold to the native state. In the native folded state, the fluorescence f...

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Detalhes bibliográficos
Main Authors: Zhuang, Xiaowei, Ha, Taekjip, Kim, Harold D., Centner, Thomas, Labeit, Siegfried, Chu, Steven
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC18902/
https://ncbi.nlm.nih.gov/pubmed/11121030
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