Fluorescence quenching: A tool for single-molecule protein-folding study

By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye molecules attached are able to fold to the native state. In the native folded state, the fluorescence f...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Zhuang, Xiaowei, Ha, Taekjip, Kim, Harold D., Centner, Thomas, Labeit, Siegfried, Chu, Steven
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 2000
Schlagworte:	Biological Sciences
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC18902/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11121030/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.26.14241
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