Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter molecules

We describe a physical mRNA mapping strategy employing fluorescent self-quenching reporter molecules (SQRMs) that facilitates the identification of mRNA sequence accessible for hybridization with antisense nucleic acids in vitro and in vivo, real time. SQRMs are 20–30 base oligodeoxynucleotides with...

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Detalhes bibliográficos
Main Authors: Gifford, Lida K., Opalinska, Joanna B., Jordan, David, Pattanayak, Vikram, Greenham, Paul, Kalota, Anna, Robbins, Michelle, Vernovsky, Kathy, Rodriguez, Lesbeth C., Do, Bao T., Lu, Ponzy, Gewirtz, Alan M.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2005
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC549423/
https://ncbi.nlm.nih.gov/pubmed/15718294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni024
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