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Improved transposon-based library preparation for the Ion Torrent platform

A transposon-based approach for the construction of sequencing libraries is an effcient way of preparing samples for processing on both Illumina and Ion Torrent platforms. However, PCR-mediated incorporation of adaptors in tagged DNA fragments leaves behind self-complementary regions fanking the DNA...

詳細記述

保存先:
書誌詳細
出版年:Biotechniques
主要な著者: Gorbacheva, Tatyana, Quispe-Tintaya, Wilber, Popov, Vasily N., Vijg, Jan, Maslov, Alexander Y.
フォーマット: Artigo
言語:Inglês
出版事項: 2015
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5494161/
https://ncbi.nlm.nih.gov/pubmed/25861933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114277
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