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Improved transposon-based library preparation for the Ion Torrent platform

A transposon-based approach for the construction of sequencing libraries is an effcient way of preparing samples for processing on both Illumina and Ion Torrent platforms. However, PCR-mediated incorporation of adaptors in tagged DNA fragments leaves behind self-complementary regions fanking the DNA...

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Bibliografiske detaljer
Udgivet i:Biotechniques
Main Authors: Gorbacheva, Tatyana, Quispe-Tintaya, Wilber, Popov, Vasily N., Vijg, Jan, Maslov, Alexander Y.
Format: Artigo
Sprog:Inglês
Udgivet: 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5494161/
https://ncbi.nlm.nih.gov/pubmed/25861933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114277
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