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Investigation of Mg(2+)- and temperature-dependent folding of the hairpin ribozyme by photo-crosslinking: effects of photo-crosslinker tether length and chemistry

We have used photo-crosslinking to investigate the structure and dynamics of four-way junction hairpin ribozyme constructs. Four phenylazide photo-crosslinkers were coupled to 2′-NH(2)-modified U+2 in the substrate and irradiated at different Mg(2+) concentrations and temperatures. Consistent with t...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Borda, Emily J., Sigurdsson, Snorri Th.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 2005
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC549404/
https://ncbi.nlm.nih.gov/pubmed/15722480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki237
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