Investigation of Mg(2+)- and temperature-dependent folding of the hairpin ribozyme by photo-crosslinking: effects of photo-crosslinker tether length and chemistry

We have used photo-crosslinking to investigate the structure and dynamics of four-way junction hairpin ribozyme constructs. Four phenylazide photo-crosslinkers were coupled to 2′-NH(2)-modified U+2 in the substrate and irradiated at different Mg(2+) concentrations and temperatures. Consistent with t...

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Udgivet i:Nucleic Acids Res
Main Authors: Borda, Emily J., Sigurdsson, Snorri Th.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2005
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Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC549404/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15722480/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gki237
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