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Investigation of Mg(2+)- and temperature-dependent folding of the hairpin ribozyme by photo-crosslinking: effects of photo-crosslinker tether length and chemistry
We have used photo-crosslinking to investigate the structure and dynamics of four-way junction hairpin ribozyme constructs. Four phenylazide photo-crosslinkers were coupled to 2′-NH(2)-modified U+2 in the substrate and irradiated at different Mg(2+) concentrations and temperatures. Consistent with t...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2005
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC549404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15722480/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gki237 |
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