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Characterization of aminoacyl-tRNA synthetase stability and substrate interaction by differential scanning fluorimetry
Differential scanning fluorimetry (DSF) is a fluorescence-based assay to evaluate protein stability by determining protein melting temperatures. Here, we describe the application of DSF to investigate aminoacyl-tRNA synthetase (AARS) stability and interaction with ligands. Employing three bacterial...
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| Pubblicato in: | Methods |
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| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5493479/ https://ncbi.nlm.nih.gov/pubmed/27794454 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.10.013 |
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