Characterization of aminoacyl-tRNA synthetase stability and substrate interaction by differential scanning fluorimetry

Differential scanning fluorimetry (DSF) is a fluorescence-based assay to evaluate protein stability by determining protein melting temperatures. Here, we describe the application of DSF to investigate aminoacyl-tRNA synthetase (AARS) stability and interaction with ligands. Employing three bacterial...

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Udgivet i:Methods
Main Authors: Abbott, Jamie A., Livingston, Nathan M., Egri, Shawn B., Guth, Ethan, Francklyn, Christopher S.
Format: Artigo
Sprog:Inglês
Udgivet: 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5493479/
https://ncbi.nlm.nih.gov/pubmed/27794454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.10.013
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