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Direct measurement of oligonucleotide substrate binding to wild-type and mutant ribozymes from Tetrahymena.

Like protein enzymes, RNA enzymes (ribozymes) provide specific binding sites for their substrates. We now show that equilibrium dissociation constants for complexes between the Tetrahymena ribozyme and its RNA substrates and products can be directly measured by electrophoresis in polyacrylamide gels...

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Detaylı Bibliyografya
Asıl Yazarlar: Pyle, A M, McSwiggen, J A, Cech, T R
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1990
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC54920/
https://ncbi.nlm.nih.gov/pubmed/2236030
Etiketler: Etiketle
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