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An effective and efficient method of transfecting primary human chondrocytes in suspension
Human chondrocytes accumulate an ECM-rich matrix by secreting matrix macromolecules during monolayer culture, which makes them difficult to transfect efficiently. Here we report a non-viral based protocol to transfect the primary human chondrocytes with high efficiency in suspension. Chondrocyte cul...
Gardado en:
| Publicado en: | Anal Biochem |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5490990/ https://ncbi.nlm.nih.gov/pubmed/28315317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2017.03.009 |
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