Identification of human antibody fragment clones specific for tetanus toxoid in a bacteriophage lambda immunoexpression library.
We have applied a molecular biology approach to the identification of human monoclonal antibodies. Human peripheral blood lymphocyte mRNA was converted to cDNA and a select subset was amplified by the polymerase chain reaction. These products, containing coding sequences for numerous immunoglobulin...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1990
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54899/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2146680/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.20.8095 |
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