Identification of human antibody fragment clones specific for tetanus toxoid in a bacteriophage lambda immunoexpression library.

We have applied a molecular biology approach to the identification of human monoclonal antibodies. Human peripheral blood lymphocyte mRNA was converted to cDNA and a select subset was amplified by the polymerase chain reaction. These products, containing coding sequences for numerous immunoglobulin...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Mullinax, R L, Gross, E A, Amberg, J R, Hay, B N, Hogrefe, H H, Kubitz, M M, Greener, A, Alting-Mees, M, Ardourel, D, Short, J M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54899/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2146680/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.20.8095
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