EcoBLMcrX, a classical modification-dependent restriction enzyme in Escherichia coli B: Characterization in vivo and in vitro with a new approach to cleavage site determination

Here we characterize the modification-dependent restriction enzyme (MDE) EcoBLMcrX in vivo, in vitro and in its genomic environment. MDE cleavage of modified DNAs protects prokaryote populations from lethal infection by bacteriophage with highly modified DNA, and also stabilizes lineages by reducing...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Fomenkov, Alexey, Sun, Zhiyi, Dila, Deborah K., Anton, Brian P., Roberts, Richard J., Raleigh, Elisabeth A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5487053/
https://ncbi.nlm.nih.gov/pubmed/28654677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179853
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