HEK293T cell lines defective for O-linked glycosylation

Here we describe derivatives of the HEK293T cell line that are defective in their ability to generate mucin-type O-linked glycosylation. Using CRISPR/Cas9 and a single-cell GFP-sorting procedure, the UDP-galactose-4-epimerase (GALE), galactokinase 1 (GALK1), and galactokinase 2 (GALK2) genes were kn...

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Udgivet i:PLoS One
Main Authors: Termini, James M., Silver, Zachary A., Connor, Bryony, Antonopoulos, Aristotelis, Haslam, Stuart M., Dell, Anne, Desrosiers, Ronald C.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2017
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5487050/
https://ncbi.nlm.nih.gov/pubmed/28654657
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179949
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