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Expression, Purification and Crystallisation of the Adenosine A(2A) Receptor Bound to an Engineered Mini G Protein

G protein-coupled receptors (GPCRs) promote cytoplasmic signalling by activating heterotrimeric G proteins in response to extracellular stimuli such as light, hormones and nucleosides. Structure determination of GPCR–G protein complexes is central to understanding the precise mechanism of signal tra...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Carpenter, Byron, Tate, Christopher G.
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5484405/
https://ncbi.nlm.nih.gov/pubmed/28660236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2234
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