Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination.

We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider spectrum of designed genomic alterations is now feasible. As a first application, we inserted a 5.4-kilobas...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Mansour, S L, Thomas, K R, Deng, C X, Capecchi, M R
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54813/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2120706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.19.7688
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