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Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination.

We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider spectrum of designed genomic alterations is now feasible. As a first application, we inserted a 5.4-kilobas...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Mansour, S L, Thomas, K R, Deng, C X, Capecchi, M R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1990
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54813/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2120706/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.19.7688
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