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Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion

The genetic code in a eukaryotic system has been expanded by the engineering of Escherichia coli tyrosyl-tRNA synthetase (TyrRS) with the Y37V and Q195C mutations (37V195C), which specifically recognize 3-iodo-l-tyrosine rather than l-tyrosine. In the present study, we determined the 3-iodo-l-tyrosi...

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Detalhes bibliográficos
Main Authors: Kobayashi, Takatsugu, Sakamoto, Kensaku, Takimura, Tetsuo, Sekine, Ryo, Vincent, Kelly, Kamata, Kenji, Nishimura, Susumu, Yokoyama, Shigeyuki
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC547826/
https://ncbi.nlm.nih.gov/pubmed/15671170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0407039102
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