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Mechanism of NTP Binding to the Active Site of T7 RNA Polymerase Revealed by Free-Energy Simulation

In genetic transcription, molecular dynamic details and energetics of NTP binding to the active site of RNA polymerase (RNAP) are poorly understood. In this article, we investigated the NTP binding process in T7 RNAP using all-atom MD simulation combined with the umbrella sampling technique. Based o...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Wu, Shaogui, Li, Laicai, Li, Quan
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5474740/
https://ncbi.nlm.nih.gov/pubmed/28591598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2017.04.039
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