Multiplexed Spliced-Leader Sequencing: A high-throughput, selective method for RNA-seq in Trypanosomatids

High throughput sequencing techniques are poorly adapted for in vivo studies of parasites, which require prior in vitro culturing and purification. Trypanosomatids, a group of kinetoplastid protozoans, possess a distinctive feature in their transcriptional mechanism whereby a specific Spliced Leader...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Cuypers, Bart, Domagalska, Malgorzata A., Meysman, Pieter, Muylder, Géraldine de, Vanaerschot, Manu, Imamura, Hideo, Dumetz, Franck, Verdonckt, Thomas Wolf, Myler, Peter J., Ramasamy, Gowthaman, Laukens, Kris, Dujardin, Jean-Claude
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5473914/
https://ncbi.nlm.nih.gov/pubmed/28623350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03987-0
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