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Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

BACKGROUND: The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simultaneously quantitate production of other recombinant proteins by transcript...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Puckette, Michael, Burrage, Thomas, Neilan, John G., Rasmussen, Max
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5469038/
https://ncbi.nlm.nih.gov/pubmed/28606077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-017-0367-0
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