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Nanomechanics of the substrate binding domain of Hsp70 determine its allosteric ATP-induced conformational change

Owing to the cooperativity of protein structures, it is often almost impossible to identify independent subunits, flexible regions, or hinges simply by visual inspection of static snapshots. Here, we use single-molecule force experiments and simulations to apply tension across the substrate binding...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Mandal, Soumit Sankar, Merz, Dale R., Buchsteiner, Maximilian, Dima, Ruxandra I., Rief, Matthias, Žoldák, Gabriel
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5468673/
https://ncbi.nlm.nih.gov/pubmed/28533394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1619843114
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