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Nanomechanics of the substrate binding domain of Hsp70 determine its allosteric ATP-induced conformational change

Owing to the cooperativity of protein structures, it is often almost impossible to identify independent subunits, flexible regions, or hinges simply by visual inspection of static snapshots. Here, we use single-molecule force experiments and simulations to apply tension across the substrate binding...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Mandal, Soumit Sankar, Merz, Dale R., Buchsteiner, Maximilian, Dima, Ruxandra I., Rief, Matthias, Žoldák, Gabriel
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5468673/
https://ncbi.nlm.nih.gov/pubmed/28533394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1619843114
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