Repeat-Length-Independent Broad-Spectrum Shuffling, a Novel Method of Generating a Random Chimera Library In Vivo

We describe a novel method of random chimeragenesis based on highly frequent deletion formation in the Escherichia coli ssb-3 strain and a deletion-directed chimera selection system that uses the rpsL(+) gene as a reporter. It enables the selection of chimeras without target gene expression and can...

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Detalhes bibliográficos
Main Authors: Mori, Koichi, Mukaihara, Takafumi, Uesugi, Yoshiko, Iwabuchi, Masaki, Hatanaka, Tadashi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC546693/
https://ncbi.nlm.nih.gov/pubmed/15691927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.754-760.2005
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