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Change in Substrate Preference of Streptomyces Aminopeptidase through Modification of the Environment around the Substrate Binding Site

We attempted to alter the substrate preference of aminopeptidase from Streptomyces septatus TH-2 (SSAP). Because Asp198 and Phe221 of SSAP are located in the substrate binding site, we screened 2,000 mutant enzymes with D198X/F221X mutations. By carrying out this examination, we obtained two enzymes...

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Autors principals: Arima, Jiro, Uesugi, Yoshiko, Iwabuchi, Masaki, Hatanaka, Tadashi
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1694256/
https://ncbi.nlm.nih.gov/pubmed/17028223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01460-06
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