Alteration of Leucine Aminopeptidase from Streptomyces septatus TH-2 to Phenylalanine Aminopeptidase by Site-Directed Mutagenesis

To tailor leucine aminopeptidase from Streptomyces septatus TH-2 (SSAP) to become a convenient biocatalyst, we are interested in Phe221 of SSAP, which is thought to interact with the side chain of the N-terminal residue of the substrate. By using saturation mutagenesis, the feasibility of altering t...

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Detalhes bibliográficos
Main Authors: Arima, Jiro, Uesugi, Yoshiko, Iwabuchi, Masaki, Hatanaka, Tadashi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1287679/
https://ncbi.nlm.nih.gov/pubmed/16269763
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.11.7229-7235.2005
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