Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts

Genetically modified pigs have important roles in agriculture and biomedicine. However, genome-specific knock-in techniques in pigs are still in their infancy and optimal strategies have not been extensively investigated. In this study, we performed electroporation to introduce a targeting donor vec...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Xie, Zicong, Pang, Daxin, Wang, Kankan, Li, Mengjing, Guo, Nannan, Yuan, Hongming, Li, Jianing, Zou, Xiaodong, Jiao, Huping, Ouyang, Hongsheng, Li, Zhanjun, Tang, Xiaochun
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5465212/
https://ncbi.nlm.nih.gov/pubmed/28596588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-02785-y
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