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Incorporation of Fluorescence Ceramide-Based HPLC Assay for Rapidly and Efficiently Assessing Glucosylceramide Synthase In Vivo

Glucosylceramide synthase (GCS) is a rate-limiting enzyme catalyzing ceramide glycosylation, thereby regulating cellular ceramide levels and the synthesis of glycosphingolipids (GSLs) in cellular membranes. Alterations of GCS not only affect membrane integrity, but also closely correlate with stem c...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Khiste, Sachin K., Hosain, Salman B., Dong, Yixuan, Uddin, Mohammad B., Roy, Kartik R., Hill, Ronald A., Liu, Zhijun, Liu, Yong-Yu
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5462733/
https://ncbi.nlm.nih.gov/pubmed/28592871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03320-9
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