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Site-specific labeling of the ribosome for single-molecule spectroscopy

Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach requires site-specific and stable attachment of fluorophores to the components of translation machinery....

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Main Authors: Dorywalska, Magdalena, Blanchard, Scott C., Gonzalez, Ruben L., Kim, Harold D., Chu, Steven, Puglisi, Joseph D.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2005
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC546139/
https://ncbi.nlm.nih.gov/pubmed/15647501
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki151
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