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Tetracycline-inducible and reversible stable gene expression in human iPSC-derived neural progenitors and in the postnatal mouse brain

The pB-tet-GOI plasmid system allows for stable piggyBac transposition-mediated integration into cells, a fluorescent nuclear reporter to identify cells that have been transfected, and robust transgene activation or suppression upon the addition of dox to the cell culture or diet of the animal. Furt...

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Bibliografski detalji
Izdano u:Curr Protoc Stem Cell Biol
Glavni autori: Akhtar, Aslam Abbasi, Breunig, Joshua J.
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5458779/
https://ncbi.nlm.nih.gov/pubmed/28510333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpsc.28
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