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Performing Yeast One-Hybrid Library Screens
Yeast one-hybrid (Y1H) assays are used to identify which transcription factor (TF) “prey” molecules can bind a DNA fragment of interest that is used as “bait”. Y1H assays involve introducing plasmids that encode TFs into a yeast “bait strain” in which the DNA fragment of interest is integrated upstr...
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| Veröffentlicht in: | Cold Spring Harb Protoc |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5458601/ https://ncbi.nlm.nih.gov/pubmed/27934684 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot088955 |
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