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Performing Yeast One-Hybrid Library Screens

Yeast one-hybrid (Y1H) assays are used to identify which transcription factor (TF) “prey” molecules can bind a DNA fragment of interest that is used as “bait”. Y1H assays involve introducing plasmids that encode TFs into a yeast “bait strain” in which the DNA fragment of interest is integrated upstr...

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Podrobná bibliografie
Vydáno v:Cold Spring Harb Protoc
Hlavní autoři: Fuxman Bass, Juan I., Reece-Hoyes, John S., Walhout, Albertha J.M.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5458601/
https://ncbi.nlm.nih.gov/pubmed/27934684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot088955
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