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Colony Lift Colorimetric Assay for β-Galactosidase Activity

In this protocol, we present a qualitative assay for monitoring the level of expression of β-galactosidase, an enzyme encoded by the LacZ gene, in yeast. This is useful both for determining autoactivity of LacZ expression in yeast DNA “bait” strains and for assessing LacZ reporter gene activation me...

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Detalhes bibliográficos
Publicado no:Cold Spring Harb Protoc
Main Authors: Fuxman Bass, Juan I., Reece-Hoyes, John S., Walhout, Albertha J.M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5458599/
https://ncbi.nlm.nih.gov/pubmed/27934685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot088963
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